Genetic Variation in Zea mays Influences Microbial Nitrification and Denitrification in Conventional Agroecosystems

Themes: Sustainability

Keywords: Genetics, Roots, Soil

Citation

Favela, A., Bohn, M.O., Kent, A. May 18, 2024. Data from: “Genetic Variation in Zea mays Influences Microbial Nitrification and Denitrification in Conventional Agroecosystems.” GitHub Repository.

Overview

PerMANOVA results for the overall microbiome (16S rRNA) and different nitrogen cycling functional genes included in this study (nitrification: *AamoA* – archaeal *amoA* [ammonia monooxygenase], *BamoA* – bacterial *amoA*; denitrification: *nirS* and *nirK* – nitrite reductase, *nosZ* – nitrous oxide reductase). The y-axis shows R.2, percent variance explained by the treatment factor, and the x-axis shows the functional genes tested. (* – P < 0.05, ** – P < 0.01, *** – P < 0.001)

Supplemental sequence processing and R statistical analysis for publication which compares the microbiome of 27 Zea cultivars: 12 Inbred maize genotypes, 9 hybrids, and 6 wild teosinte. The project contains amplicon data for various genes: 16S rRNA, ITS, bacterial amoA, Archeal amoA, nirS, nirK, and nosZ. In addition to functional potential assay data, and N2O flux.

Data

GitHub: Scripts (Shell, R, Perl)

NIH – NCBI BioProject: Rhizosphere microbiome from Maize cultivars

Illinois Data Bank includes:

Primers
Genotype and classification summaries
Bacterial, nirS, nosZ, Archael, nirK models

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