Comparing Four Heat-inducible Promoters in Stably Transformed Sugarcane Regarding Spatial and Temporal Control of Transgene Expression Reveals Candidates to Drive Stem-preferred Transgene Expression

Themes: Feedstock Production

Keywords: Gene Expression, RNA Sequencing, Software, Sugarcane

Citation

Qiande, M., Altpeter, F. Oct. 30, 2025. Data for: “Comparing Four Heat-inducible Promoters in Stably Transformed Sugarcane Regarding Spatial and Temporal Control of Transgene Expression Reveals Candidates to Drive Stem-preferred Transgene Expression.” Zenodo. DOI: 10.5281/zenodo.17486962.

Overview

GUS activities in HSP lines and WT after heat treatment compared with that in Ubi lines in different stem sections (top, middle and base of stem). (a) pGmHSP17.5 lines. (b) pZmHSP17.7 lines. pHvHSP17 and pZmHSP26 not shown. uidA copy numbers are shown in parentheses following the line IDs. Different letters indicate significant difference at p < 0.05 according to Fisher‘s least significant difference (LSD) comparison.

Raw data for publication:  Moni Qiande and Fredy Altpeter. 2025. Comparing four heat-inducible promoters in stably transformed sugarcane regarding spatial and temporal control of transgene expression reveals candidates to drive stem preferred transgene expression. Frontiers in Plant Science (accepted for publication). The dataset includes the raw data from quantitative PCR of MUG assays of transgenic sugarcane

Data

Zenodo:  Raw leaf, stem, and root data

GitHub: Python code for a DNA/RNA sequence pattern finder

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