Variability in Structural Carbohydrates, Lipid Composition, and Cellulosic Sugar Production from Industrial Hemp Varieties

Themes: Conversion, Feedstock Production

Keywords: Biomass Analytics, Feedstock Bioprocessing

Citation

Viswanathan, M.B., Park, K., Cheng, M.H., Cahoon, E.B., Dweikat, I., Clemente, T., Singh, V. Sept. 2, 2020. “Variability in Structural Carbohydrates, Lipid Composition, and Cellulosic Sugar Production from Industrial Hemp Varieties.” Industrial Crops and Products 157, 112906. DOI: 10.1016/j.indcrop.2020.112906.

Overview

Relative amount of triacylglycerol (TAG) from total lipids was calculated from GC analysis. CBD hemp was the most TAG-rich hemp variety (~25% of total lipid), whereas TAG comprised only ~2% of the total lipid in 19m hemp.

The aim of this study was to determine carbohydrate recovery from hemp for ethanol production and quantify biodiesel from TAG (triacylglycerol) present in hemp. The structural composition of five different hemp varieties (Seward County-SC, York County-YC, Loup County-LC, 19 m96136-19 m, and CBD Hemp-CBD) were analyzed. Concentration of glucan and xylan ranged between 32.63 to 44.52% and 10.62 to 15.48% respectively. The biomass was then pretreated with Liquid hot water followed by disk milling and then hydrolyzed enzymatically to yield monomeric sugars. High glucose (63-85%) and xylose (73-88%) recovery was achieved. Lipids were extracted from hemp using hexane and isopropanol and then transesterified to produce biodiesel. Approximately, 50% of total fatty acids in SC, LC, and CBD hemp were linoleic acid. Palmitic acid was present between 32 to 50% in varieties YC and 19 m. Highest TAG concentration at 25% of total lipids was observed in CBD hemp. The analysis on lipid composition and high sugar recovery demonstrates hemp as a potential bioenergy crop for ethanol and biodiesel coproduction.

Data

Download (4KB) includes:

  • Plant growth and yield
  • Hemp compositional analysis
  • FAME composition
  • Pretreatment inhibitors
  • Sugar yield