Transformation and Gene Editing in the Bioenergy Grass Miscanthus

Themes: Feedstock Production

Keywords: Biomass Analytics, Genomics

Citation

Trieu, A.Belaffif, M.Hirannaiah, P.Manjunatha, S., Wood, R., Bathula, Y., Billingsley, R.L.Arpan, A.Sacks, E.J.Clemente, T.E.Moose, S.P.Reichert, N.A.Swaminathan, K. Dec 28, 2022. “Transformation and Gene Editing in the Bioenergy Grass Miscanthus.” Biotechnology for Biofuels and Bioproducts 15 (148). DOI: 10.1186/s13068-022-02241-8.

Overview

Leaf phenotypes in five Miscanthus genotypes transformed with gene editing vector pHA194. Examples of altered leaf colors (pale green/yellow, striped, white) noted in Miscanthus regenerants arising from embryogenic calli transformed with pHA194 (contained Cas9 gene and three gRNA targeting lw1). Five genotypes are represented, two transformed via biolistics (Msa S1, Msi UI1) and three via A. tumefaciens (Msa S13, Msi P1, Mxg Freedom). WT = isogenic wild-type line, lw1 = transgenic edited shoots/plantlets/plants

Miscanthus, a C4 member of the family Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies Miscanthus x giganteus and its parental species M. sacchariflorus and M. sinensis. Use of biotechnology-based procedures to genetically improve miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Data

Download (21.9 KB) includes:

  • Lemonwhite gRNAs
  • Miscanthus lines
  • Culture Initiation
  • Media Additives