Themes: Conversion

Keywords: Genome Engineering, Software


Enghiad, B.Xue, P.Boob, A.Singh, N.Shi, C.Lane, S.T.Petrov, V.A., Liu, R., Peri, S.S., Gaither, E.D.Zhao, H. Dec. 31, 2021. Data from: “PlasmidMaker: A Versatile, Automated, and High Throughput End-to-End Platform for Plasmid Construction.” Zenodo. DOI: 10.5281/zenodo.5812313.


PlasmidMaker: Computer-assisted plasmid design and robotic assembly.

Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-consuming, labor-intensive, and rate-limiting steps in performing sophisticated biological experiments. Here, we report the development of a versatile, robust, automated end-to-end platform named PlasmidMaker that allows error-free construction of plasmids with virtually any sequences in a high-throughput manner. This platform consists of a most versatile DNA assembly method using Pyrococcus furiosus Argonaute (PfAgo)-based artificial restriction enzymes, a user-friendly frontend for plasmid design, and a backend that streamlines the workflow and integration with a robotic system. As a proof of concept, we used this platform to generate ~100 plasmids from six different species ranging from 5 to 18 kb in size from up to 11 DNA fragments within three days. PlasmidMaker should greatly expand the potential of synthetic biology.


PlasmidMaker Web Application

Frontend and Backend Code

Download (7.3 KB) includes:

  • 101 Plasmid DNA Sequences (.csv format)
  • Primer and Guide DNA Sequences (.dna format)
  • API Endpoint Guide

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