Harnessing Photoenzymatic Reactions for Unnatural Biosynthesis in Microorganisms
Themes: Conversion
Keywords: Bioproducts, Catalysis, Metabolic Engineering
Citation
Yuan, Y., Li, M., Harrison, W., Zhang, Z., Zhao, H. Dec. 10, 2025. Data for: “Harnessing Photoenzymatic Reactions for Unnatural Biosynthesis in Microorganisms.” Dryad. DOI: 10.5061/dryad.ns1rn8q54
Overview
This dataset supports the research published in “Harnessing Photoenzymatic Reactions for Unnatural Biosynthesis in Microorganisms”. It includes all the necessary SnapGene files for the plasmids described in the article and the Supplementary Information. All files are provided in SnapGene (.dna) format and can be opened and edited using SnapGene or SnapGene Viewer.
- OYE family enzymes (Old Yellow Enzymes / ene-reductases)
- Auxiliary NADPH-regeneration systems
- Promoter comparisons (T7, Trc, lac)
- 4-vinylphenol and styrene biosynthetic pathway construction
- Photobiocatalysis pathways (using photoenzymes + NADPH regeneration)
- Whole-cell screening of OYE mutants
Data
Dryad: Plasmid SnapGene files, promoter comparisons, pathways