
University of Illinois 2022 iGEM team members from left: Adrianne Adaya, Airah Zaidi, Angela Wang, Melissa Chin, Luren Wang, Megan Zhou, and Vishnu Kompella.
iGEM 2022 Team: Seeking Solution to the Nitrate Problem
EDITOR’S UPDATE: The Illinois team’s “Curli Capture” was awarded a bronze medal at the International Genetically Engineered Machine (iGEM) 2022 competition. The seven-member student team worked to design proteins that can help remediate waterways suffering from the ill effects of nitrate runoff.
Modern agriculture has become a major source of pollution in many forms. One facet of this pollution is nitrate runoff. Nitrates are used in fertilizers as a source of nitrogen, which is essential for plant growth. Although these fertilizers help improve crop yields, excess nitrates often end up in waterways, where they can cause harmful algal blooms and other ecological problems.
Perhaps the most well-known example of the ill effects of nutrient runoff is the Gulf of Mexico dead zone. Excess fertilizer in the Mississippi River watershed washes downstream and into the gulf. The abundance of nutrients fuels the rapid growth of algae, which depletes oxygen availability. This is known as hypoxia — oxygen levels drop so low that they can no longer sustain marine life.
Closer to home in Illinois, Lake Decatur supplies 39.4 million gallons of water every day to 87,000 people in surrounding communities. However, due to agricultural runoff, high concentrations of nitrate can be found in the lake, creating unsafe drinking water conditions and disruptions in the aquatic ecosystem by overstimulating plant growth.
Enter the 2022 University of Illinois iGEM team, who kicked off their research with a single guiding question: How can nitrate runoff be remediated to improve the ecological health of our waterways?
This team of seven U of I undergraduates and their graduate student and faculty mentors from the University of Illinois Urbana-Champaign was sponsored by the Carl R. Woese Institute for Genomic Biology (IGB) and CABBI to take part in the annual International Genetically Engineered Machine (iGEM) Competition in Summer 2022.

A bacterium can be engineered to secrete self-assembling curli fibers with the ability to capture environmental pollutants.
Their project, called Curli Capture, offers a possible solution to our nitrate pollution problem. Inspired by previous synthetic biology work on engineering catalytic biofilms to capture heavy metals, the iGEM team planned to engineer a biofilm that can capture excess nitrate in bodies of water — a solution with the potential to be cost-effective and environmentally friendly.
By fusing a nitrate-binding protein to curli biofilm, a nitrate-capturing system can be created. Curli biofilm is a collection of fibrous proteins woven together like a scaffolding, which can be used to support nitrate-binding proteins that bind to and capture nitrate. The curli biofilm and nitrate-binding protein can be permanently attached to each other via a synthetic biology technology called the SpyTag/SpyCatcher system.
To actualize this Curli Capture system, the Illinois iGEM team engineered bacteria that could produce the necessary components. This was achieved by inserting specially crafted plasmids into E. coli. Plasmids are small circular DNA molecules that can be used to introduce new genes into an organism. The first plasmid included the genetic sequence that codes for the curli protein and the SpyTag sequence, which codes for a short chain of amino acids. The second plasmid contained the sequence that codes for the nitrate-binding protein and the SpyCatcher sequence, which codes for the SpyCatcher protein that will bond to the SpyTag protein when the two come into contact. The interaction between SpyCatcher and Spytag will securely attach the nitrate-binding protein to the curli biofilm.
Though the team was unable to fully characterize and recombine their engineered strains, they remain hopeful that such a system could be deployed in the future as a means of reducing nitrate pollution in agricultural runoff. Additionally, they noted that by fusing different functional proteins to curli biofilms, systems similar to Curli Capture could be engineered for the remediation of other pollutants as well.
The iGEM team consists of Adrianne Adaya, junior in Chemical Engineering; Airah Zaidi, junior in Chemical & Biomolecular Engineering; Vishnu Kompella, sophomore in Physics; Angela Wang, junior in Biochemistry; Megan Zhou, junior in Bioengineering; Melissa Chin, junior in Chemical Engineering; and Luren Wang, undergrad in Mechanical Engineering. For many, this was their first experience designing a synthetic biology research project. The students are optimistic that the skills they’ve built up from this project will help them reach new heights as they continue to pursue their research interests.
This year’s iGEM mentors are Chris Rao, professor of Chemical and Biomolecular Engineering; Ebin Joseph, Ph.D. student in Chemical and Biomolecular Engineering; Anshu Deewan, Ph.D. student in Chemical and Biomolecular Engineering; William Woodruff, Ph.D. student in Chemical Engineering; and Michael Volk, Ph.D. student in Chemical and Biomolecular Engineering.
On Oct. 26-28, the Illinois iGEM team will present its work remotely at the 2022 iGEM Grand Jamboree, a combined remote/in-person celebration of the research accomplishments of over 350 iGEM teams from around the world.
Read more about Curli Capture and the team on its webpage >>>
— Article by CABBI Communications Specialist April Wendling
Read about the 2021 iGEM team
University of Illinois iGEM Team 2021 Brings Home Bronze Medal
EDITOR’S UPDATE: The Illinois team’s “Project apPETite” was awarded a bronze medal at the International Genetically Engineered Machine (iGEM) 2021 competition. The six-member student team worked to design better enzymes to break down widely used Polyethylene terephthalate (PET) plastics. Learn more in this two-minute video or read our August story about the project below.
CABBI and the Carl R. Woese Institute for Genomic Biology (IGB) are sponsoring six undergraduate students from the University of Illinois Urbana-Champaign to take part in the International Genetically Engineered Machine (iGEM) Competition during Spring and Summer 2021. The annual iGEM Jamboree is in the fall.
Polyethylene terephthalate, or PET, is a type of plastic that is widely used for packaging food and beverages, including soft drinks, juices, and water. Although PET is the most recycled plastic in the U.S., its current recycling rate is only 31%. The 2021 Illinois iGEM team aims to improve that by tweaking PETase — a naturally occurring enzyme found in Ideonella sakaiensis, a bacterium discovered in 2016 as the world’s first PET-eating bacterium.
PET is usually commercially recycled by re-melting or with the help of chemicals. Each method has disadvantages. Although incinerating PET bottles is cheaper and more efficient, it slowly releases CO2. On the other hand, the chemical treatments make PET resistant to biological degradation. Additionally, only clean PET plastic waste, without any residuals from food or drinks, can be treated by the current industrial PETase recycling methods, necessitating the use of alternative treatment procedures.
The current PETase enzyme made by I. sakaiensis cannot be used for many of the existing recycling conditions.
“The majority of PET ends up at landfills. Since it is very hard to degrade PET naturally, we are looking at PETase and trying to optimize how it degrades the plastic,” said Mary Cook, a senior in bioengineering.
The team has a two-pronged approach to the problem: the computational team that will design new enzymatic sequences; and the wet lab team that will create and test these new mutated proteins for degradation.
“We are using the published models that have previously looked at PETase to try to figure out which amino acid sequences contribute to better enzyme activity,” said Suva Narayan, a senior in bioengineering.
On the other hand, the wet lab team has started to test the wild-type PETase to understand the enzyme better.
“Once the computational team tells us which mutations to use, we’re going to test the different forms of the enzyme to see how effective they are at degrading PET at different temperatures,” said Royal Shrestha, a junior in molecular and cellular biology.
“We’re hoping that the computationally-derived sequences will be more promising than the wild-type PETase,” Cook said. “We want to figure out which parts of the enzyme sequence are important for certain characteristics and find something that is better at degrading PET.”
The iGEM teams usually meet in the spring to develop their ideas for synthetic biology projects and they continue working on them over the summer. They then participate at the annual Giant Jamboree, an international competition, in November. Teams from all over the world participate to earn gold, silver, or bronze medals and the overall winners are chosen from the gold-medal teams.
Some of the members in the 2021 team also participated in 2020, which has allowed them to get a jump-start on the project.
“Last year we spent a lot of time figuring out what our project was going to be. This year, we formed our team earlier and started working in May instead of later in the summer,” Cook said. “iGEM is unique because it is completely student driven. So, it was nice to have a little extra time to figure out our direction.”
Last year, the iGEM team built a web tool to model the COVID “spike” protein as it mutates to enable the design of new drugs and vaccines. Intriguingly, their current project idea has also stemmed from the pandemic.
“During the pandemic, the consumption of single-use plastic surged and we became interested in solving that problem.” said Angela Yoon, a senior in integrative biology. “It’s an extension of how COVID affects our lives.”
The team is also hoping to use their research to help the Urbana-Champaign community.
“Being involved in your community is a really big part of iGEM,” Cook said. “We want to reach out to local places in Champaign to get a better idea of how plastic is recycled and how we can apply our solutions here.”
The 2021 team consists of Cook; Narayan; Shrestha; Yoon; Jefrin Joseph, a junior in molecular and cellular biology; and Kristin Lai, a senior in bioengineering. They will also be partnering with the University of Toronto and the University of Texas, Austin since the iGEM teams from those universities are also working on PET plastic.
The Illinois iGEM team’s webpage >>>
— Article by iGB Science Writer Ananya Sen
Read about the 2020 iGEM team
CABBI and the Carl R. Woese Institute for Genomic Biology (IGB) sponsored six undergraduate students from the University of Illinois at Urbana-Champaign to take part in the International Genetically Engineered Machine (iGEM) Competition during Spring and Summer 2020. The annual iGEM Jamboree was online in November.
EDITOR’S UPDATE: The Illinois team’s “Viralizer” web tool brought home a silver medal at the International Genetically Engineered Machine (iGEM) 2020 competition. Working virtually, the six-member student team created the tool to model the COVID-19 spike protein as it mutates and chart its spread, to aid in the development of new drugs & vaccines.
Viralizer details more than 20,000 mutated protein sequences, as well as several hundred potential antibodies designed by the team to neutralize the spike protein. A phylogenetic tree characterizes the progression of the pandemic over space and time. Mary Cook, a junior in bioengineering, said the team was happy with the silver medal and accomplished “everything we hoped for in terms of our antibody design, our phylogenetics output, and the database models uploaded on our website.”
Suva Narayen, a junior in bioengineering who worked on antibody design, said the project turned out better than expected given the amount of work required — and some late nights — as the deadline approached. “Fortunately we were able to finish on time, and the judging and poster sessions went really well for us,” Narayen said.
This year’s virtual Jamboree “definitely felt different” and presented some logistical challenges, Cook said. “However the interactive poster that we put together was a cool way to display our work and we enjoyed still being able to meet all the judges.” The team members are now close friends and hope to continue working together in some way, she said.
“The biggest takeaway from this project for me is the fact that we came up with a solution to an ongoing problem, something I haven’t really done before, Narayan said. Because the COVID-19 pandemic is still being researched, “we had no guidelines to look to if we were stuck. It encouraged me to think outside the box and collaborate with the rest of the team more effectively.”
You can watch the full 18-minute video about the team’s Viralizer project here; read our August article about the project below.
The COVID-19 pandemic created unprecedented challenges for a worldwide competition that brings high school and college students together to tackle big questions in synthetic biology.
But it also provided a unique research opportunity for the University of Illinois team competing in this year’s iGEM contest.
The six undergraduates are pooling their talents — remotely — to contribute to the fight against SARS-CoV-2, the coronavirus that causes COVID-19. They’re creating a web tool to build visual models of a key part of the virus as it mutates — specifically the infamous “spike” protein that allows it to attack human cells so easily. The hope is to give researchers crucial information about the virus as they design new drugs and vaccines.
“Our database will be real time, and it will allow researchers to upload their newly collected coronavirus sequences and get those newly mutated protein models for their research,” said Yan Luo, a sophomore in bioengineering on the Illinois team.
Mentored by graduate students and postdocs, iGEM teams meet in the spring to brainstorm ideas for innovative synthetic biology research and develop them over the summer. The projects culminate at the annual Giant Jamboree, iGEM’s international competition, to be held virtually this year in November. The event attracts teams from nearly every continent who can earn bronze, silver, or gold medals by achieving certain standards. Overall winners are chosen from the gold-medal teams.
The Illinois iGEM team is sponsored by the Carl R. Woese Institute for Genomic Biology (IGB) and the Center for Advanced Bioenergy and Bioproducts Innovation (CABBI). Illinois has competed in iGEM since 2008, earning bronze or silver medals and occasionally gold, said Christopher Rao, Professor of Chemical and Biomolecular Engineering (ChBE), who helped launch the first iGEM team and is advising this year’s squad.
Past Illinois projects included a bacterial filing cabinet and a medical biosensor. Last year’s group took an environmental turn: developing a micro-organism to degrade glyphosate, the key ingredient in Round-Up and a suspected carcinogen. Their work showed promise, and with one student returning for this year’s competition Rao hoped the 2020 team might carry on that research.
“Then COVID happened,” said Rao, who also serves as Deputy Leader of CABBI’s Conversion Theme. “There’s no way we could do hands-on training of undergraduates in this environment.”
They decided on a purely computational project, which would be far easier to manage remotely. Given the pandemic, the students wanted to do something COVID-related, Rao said.
“We all just wanted to start fresh and have our own idea,” said Mary Cook, a junior in bioengineering.
Besides Luo and Cook, the team includes Sachin Jajoo, a senior in molecular and cellular biology; Angela Yoon, a junior in integrative biology; Suva Narayan, a junior in bioengineering; and Royal Shrestha, a sophomore in biochemistry. They are mentored by CABBI researchers Matthew Waugh, a chemistry postdoc, and ChBE graduate students William Woodruff and Carl Schultz. CABBI Research Coordinator Anna Fedders and IGB Outreach Activities Coordinator Daniel Ryerson provide administrative support.
The students started work in March, after the coronavirus shutdown. They each came up with an idea for a computational project with synthetic biology applications that would fill a research need. Shrestha suggested a 3D database as an interactive way to show viral structures and help scientists learn more about SARS-CoV-2.
The team presented the idea to their mentors, who helped focus it into a useful deliverable. The students also consulted with professors across campus involved in COVID-related research, including Erik Procko in biochemistry, Mohammed El-Kebir in computer science, and two faculty members in ChBE — Huimin Zhao and Diwakar Shukla.

Image of a coronavirus spike protein. Credit: Gerd Altmann on Pixabay
Those conversations narrowed the project to spike proteins specifically, Cook said. The team also decided to try, to design an antibody with the information they collected, rather than just a database.
SARS-CoV-2 is mutating — in small ways, but enough to potentially change how its proteins bind to human receptor cells or to a vaccine, Luo said. So researchers want to fully understand the structure of all the mutated proteins, which is a complex process.
One of the most crucial is the spike protein — named for the protrusions it causes on the surface of the coronavirus — which allows the virus to penetrate human host cells and cause infection. Any mutations could alter its infectiousness or impact the efficacy of a future vaccine.
“That is the problem that we are trying to tackle,” said Luo, who is leading the project’s software aspects. “We have thousands of sequences of the spike proteins, but we don’t know their structure. We’re taking the mutated sequences and the already available models of proteins to predict the structure of the mutated spike proteins.”
Some vaccines work through a neutralizing agent that disables the virus, almost like an antivenom, by binding with it and preventing it from interacting with the human body, Luo said. Most researchers design those neutralizing agents in wet labs, testing them hundreds of times until they find the right one. But they usually work with just one strain and don’t necessarily focus on the protein structure.
There’s no current database dedicated to producing a model for the spike protein as it mutates, Luo said. Of the 8,000-plus spike proteins found on the Global Initiative on Sharing Avian Influenza Data (GISAID) platform, “a very, very small percentage have crystal structures that are known through experimentation,” Cook said.

Image of a Zika virus phylogenic tree built through a NextStrain tutorial. Credit: Mary Cook
To build their web tool, the students are using NextStrain, an open-source project that pulls together data and visualization tools for viruses and other pathogens to track the spread of outbreaks and improve the public health response. They can upload their own protein sequences, models, and geographic data to make a phylogenic tree showing different spike protein strains in each location.
In the spirit of iGEM, their tool will be open-source. A researcher who discovers a tiny “point mutation” — a change in only one or two nucleotides from the original viral genetic sequence— will be able to enter the new sequence into the tool, which could then predict whether that mutation would make a significant difference in the crystal structure and produce a 3D model of what it would look like.
Most point mutations wouldn’t have a huge effect. But researchers have already found that a variation in one amino acid on the spike protein increased the infectiousness of SARS-COV-2 a thousand-fold, allowing it to spread quickly and become the dominant strain in Europe and the United States. The team hopes to be able to predict which genetic mutations might cause problems in the future.
Cook and Yoon are working on the visualization and website components of the project. Jajoo, an iGEM veteran, is supervising administrative aspects, and Narayan and Shrestha are heading up antibody design.
They are using a popular modeling software called Py Rosetta to compare their models for thousands of spike protein variations with simulated mutations of the human antibody. The software can analyze how they bind together, indicating which antibody offers the best defense and could be developed into a vaccine or drug.
Collaborating remotely has been a challenge. The entire team has never met in person. The online meeting format made it tough initially for students to open up, Rao said, and they didn’t have the usual bonding experience of working together in the lab. While most of the students are in Champaign-Urbana or Chicago, others are several time zones away in California and South Korea. But they’ve all adapted.
“We’ve already had some successes. I think we’re on a good track,” Luo said.
The project has been an enormous learning experience, on many levels. To write the tool, the students had to learn about viruses, programming, bioinformatics, DNA sequences, protein modeling, viral structures, graphic software, and antibody design. They’ve also had to manage expenses, divvy up tasks, and help each other sort through problems.
Connecting with professors and learning about their research was especially valuable, Cook said.
“Most people just don’t realize how much effort scientists are putting in on tackling the coronavirus,” Luo said, from vaccine development to understanding the virus.
Perhaps most important: the ability to design their own project and do research from the ground up. Choosing what problem to work on, and where you can make a difference, is one of the most important — and challenging — aspects of science, Rao said, separating the “true greats” from everyone else.
“Any research that I’d participated in before has been with a graduate student or with a mentor who had the project and the goals laid out,” Cook said. “Here, we had to start from scratch. That has been probably the most rewarding part of iGEM.”
— Article by iSEE Communications Specialist Julie Wurth
Read about the 2019 iGEM team
Five undergraduate students from the University of Illinois at Urbana-Champaign took part in the International Genetically Engineered Machine (iGEM) Competition during Summer 2019.
Read about the 2018 iGEM team

UPDATE: CABBI and Carl R. Woese Institute for Genomic Biology student researchers, from left, Pranathi Karumanchi, Amie Bott, Ziyu Wang, Alex Ruzicka, and Liam Healy enjoy their time at the iGEM Giant Jamboree in Boston. The University of Illinois group was one of 107 teams to take home home bronze-level honors. Of the 343 participating teams, 114 reached the gold level and 68 achieved silver. Read the story by Emily Scott, IGB Science Writer and Outreach Specialist, on the IGB website >>>
University of Illinois iGEM Team Takes on CABBI-funded Synthetic Biology Project
UPDATE OCTOBER 2018 — CABBI and Carl R. Woese Institute for Genomic Biology (IGB) student researchers earned a bronze-level honor at the iGEM Giant Jamboree in Boston. Read the story by Emily Scott, IGB Science Writer and Outreach Specialist, on the IGB website >>>
ORIGINAL STORY PUBLISHED July 31, 2018 — This summer, a group of undergraduate students has teamed up with CABBI researchers to pursue an ambitious research project.
Their work is in preparation for the International Genetically Engineered Machine (iGEM) competition, which brings together undergraduate students from across the world to present their research in synthetic biology and compete for prizes.
This year’s team from the University of Illinois at Urbana-Champaign is made up of five students: Pranathi Karumanchi, Ziyu Wang, Liam Healy, Amie Bott, and Alexander Ruzicka.
Their project is funded by the CABBI, a collaboration between the Institute for Sustainability, Energy, and Environment (iSEE) and the Carl R. Woese Institute for Genomic Biology (IGB) that aims to develop sustainable biofuels and bioproducts.
The iGEM team is mentored by graduate and postdoctoral researchers Carl Schultz, Shekhar Mishra, and Matthew Waugh, IGB Outreach Activities Manager Courtney Fenlon, CABBI Program Manager Elizabeth Murphy, and Associate Professor of Bioengineering and CABBI scientist Ting Lu.
The idea for the team’s project came from the joint work of Ting Lu and Yong-Su Jin, a Professor of Food Science and Human Nutrition and CABBI scientist. Lu is researching lactic acid bacteria, which is used in the production of cheese and yogurt, while Jin studies baker’s yeast, which is used in baking bread. Lu and Jin want to see what a collaboration between these two organisms could achieve in the field of metabolic engineering.
Metabolic engineering involves modifying organisms to produce valuable products, such as biofuels and chemicals. Most metabolic engineering research involves working with a single species, but Lu said this has several limitations.
“When we look at microbes in nature, we know that they do not exist in a single cell or single strain,” Lu said. “Instead, they always present in the form of complex communities.”
Lu wants to investigate the ecosystem of lactic acid bacteria and baker’s yeast and find a way to use them for producing valuable products. He said evidence has shown that these two organisms often coexist naturally and can even boost each other’s production.
The iGEM team is continuing this work; it will study how lactic acid bacteria and baker’s yeast work together, testing different environments and food sources. Team members will then engineer the organisms to see if they are capable of creating a valuable product.
One of the biggest challenges the team faces is the lack of prior research in this area.
“We’re the ones that have to figure it out,” Healy said. “We’re the ones doing a lot of the failing.”
Team members have had to troubleshoot how to make an environment that would help the organisms grow together.
“Because there’s not a lot of literature to go on, right from the early stages, this project has been one of inquiry,” Mishra said.
Despite the trial and error involved in the research, the mentors said the team has made quick progress this summer.
“They’re making some discoveries that — as far as we can tell — haven’t been made so far in literature, in terms of how to actually grow these two organisms together in such a way that they’re both able to grow healthily,” Schultz said.
Lu said the upside of the project is that any discoveries and progresses will be new to the field.
The team will present its work at this year’s iGEM competition in October.
“My impression is that they’re making pretty fast progress,” Schultz said. “It’ll be exciting to see what kind of results they’re able to get by the end.”
— Written by Emily Scott, IGB Science Writer and Outreach Specialist