Production of Galactitol from Galactose by Oleaginous Yeast Rhodosporidium toruloides IFO0880

CABBI Theme: Conversion

Keywords: Genomics, Metabolomics

Citation

Jagtap, S.S., Bedekar A.A., Liu J.J., Jin, Y.S., Rao, C.V. Oct. 18, 2019. “Production of Galactitol from Galactose by the Oleaginous Yeast Rhodosporidium toruloides IFO0880.” Biotechnology for Biofuels 12:250. DOI: 10.1186/s13068-019-1586-5.

Lipid content (a, triangles) and galactitol production (b) in N-poor media with 40 g/L galactose.

Overview

Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols.

Data

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Table S1: Comparison of galactitol production rate and yield using galactose in nitrogen-rich medium (YP) and low-nitrogen medium (LNM) by R. toruloides

Table S2: Oligonucleotides used in the study

Table S3: Nucleotide sequence of codon optimized aldose reductase

Table S4: A total of 29 significant intracellular metabolites such as amino acids, sugars, phosphates, fatty acids, organic acids, nucleosides and others, identified under glucose and galactose conditions by GC-MS analysis in R. toruloidesIFO0880

Table S5: Twenty-nine metabolites with high absolute loadings as determined by PCA