Production of Galactitol from Galactose by Oleaginous Yeast Rhodosporidium toruloides IFO0880


CABBI Theme: Conversion

Keywords: Genomics, Metabolomics



Jagtap, S.S., Bedekar A.A., Liu J.J., Jin, Y.S., Rao, C.V. Oct. 18, 2019. “Production of Galactitol from Galactose by the Oleaginous Yeast Rhodosporidium toruloides IFO0880.” Biotechnology for Biofuels 12:250. DOI: 10.1186/s13068-019-1586-5.


Lipid content (a, triangles) and galactitol production (b) in N-poor media with 40 g/L galactose.


Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols.



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Table S1: Comparison of galactitol production rate and yield using galactose in nitrogen-rich medium (YP) and low-nitrogen medium (LNM) by R. toruloides

Table S2: Oligonucleotides used in the study

Table S3: Nucleotide sequence of codon optimized aldose reductase

Table S4: A total of 29 significant intracellular metabolites such as amino acids, sugars, phosphates, fatty acids, organic acids, nucleosides and others, identified under glucose and galactose conditions by GC-MS analysis in R. toruloidesIFO0880

Table S5: Twenty-nine metabolites with high absolute loadings as determined by PCA