Production of Galactitol from Galactose by Oleaginous Yeast Rhodosporidium toruloides IFO0880
CABBI Theme: Conversion
Keywords: Genomics, Metabolomics
Jagtap, S.S., Bedekar A.A., Liu J.J., Jin, Y.S., Rao, C.V. Oct. 18, 2019. “Production of Galactitol from Galactose by the Oleaginous Yeast Rhodosporidium toruloides IFO0880.” Biotechnology for Biofuels 12:250. DOI: 10.1186/s13068-019-1586-5.
Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols.
Table S1: Comparison of galactitol production rate and yield using galactose in nitrogen-rich medium (YP) and low-nitrogen medium (LNM) by R. toruloides
Table S2: Oligonucleotides used in the study
Table S3: Nucleotide sequence of codon optimized aldose reductase
Table S4: A total of 29 significant intracellular metabolites such as amino acids, sugars, phosphates, fatty acids, organic acids, nucleosides and others, identified under glucose and galactose conditions by GC-MS analysis in R. toruloidesIFO0880
Table S5: Twenty-nine metabolites with high absolute loadings as determined by PCA